BRAF V600E Immunohistochemistry (IHC)
Use
This immunohistochemical test uses a monoclonal antibody (IHC600) against mutant BRAF (V600E) to rapidly assess mutant protein expression throughout tumor tissue in conditions such as hairy cell leukemia, certain melanomas, and thyroid carcinomas. Detection of BRAF mutation serves as a strong molecular marker of poor prognosis in colorectal carcinoma and may indicate a sporadic mechanism of mismatch repair deficiency, offering clinical insight into tumor behavior and potential therapeutic direction.
Special Instructions
Not provided.
Limitations
Not provided.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
BRAF
Protein
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred. Alternatively, one unbaked, unstained slide for H&E (required) plus two to three positively charged unstained slides cut at 4–5 microns.
Storage Instructions
Use cold pack for transport; cold pack must not be in direct contact with specimen. NYS clients provide date and time of collection.