Hepatitis B Core Antigen
Use
Hepatitis B core antigen detection by immunohistochemistry (IHC) identifies the intracellular presence of the viral core antigen localized within nuclei of hepatocytes, reflecting viral infection. It is used in pathology to detect HBV infection in tissue specimens, particularly when viral replication is suspected or when serologic results are inconclusive.
Special Instructions
Two levels of service are offered: Global (full result interpretation) and Tech‑Only (stain only). Global service yields results in approximately 2 days; Tech‑Only service delivers stain results in approximately 24 hours. For NY State clients, date and time of collection must be provided. Transport should use a cold pack that does not come in direct contact with the specimen.
Limitations
Formal fixation is required—specimens not fixed in 10% formalin may yield unsatisfactory results. Archival unstained slides should be recent; age and alternative fixatives may degrade antigenicity.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
FFPE tissue block or unbaked, unstained slides (H&E plus 2–3 positively charged unstained slides, 4–5 microns)
Collection Instructions
Submit FFPE tissue block or one unbaked, unstained H&E slide (required) and two to three positively charged unstained slides cut 4–5 μm
Storage Instructions
Use cold pack for transport; cold pack must not be in direct contact with specimen; NYS clients: include date/time of collection