High Sensitivity PNH Evaluation
Use
This high-sensitivity evaluation of Paroxysmal Nocturnal Hemoglobinuria (PNH) uses flow cytometry to detect red blood cell and granulocyte PNH clones at extremely low frequencies (down to 0.01%), providing highly sensitive diagnostic capability for identifying PNH even in patients with very small clone burdens. It supports accurate detection of PNH clones to guide diagnosis and clinical decision-making in relevant hematologic conditions.
Special Instructions
Not provided.
Limitations
Bone marrow specimens are not acceptable. Lithium heparin or ACD tubes are not acceptable; only EDTA is preferred (sodium heparin acceptable). Specimens must be received within 48 hours to assure integrity and viability. Samples must be refrigerated and never frozen; cold packs should not contact specimens directly.
New York Approved
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
Result Turnaround Time
24 hours
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
1-2 mL
Minimum Volume
Not provided
Container
EDTA tube preferred; sodium heparin acceptable; lithium heparin or ACD not acceptable
Collection Instructions
Provide a recent CBC report; bone marrow specimens not acceptable
Storage Instructions
Refrigerate specimen; do not freeze; use cold pack for transport (not in direct contact with specimen)
Causes for Rejection
Lithium heparin or ACD tubes; bone marrow specimens; samples received after 48 hours; frozen samples
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | Within 48 hours accepted |