MLH1
Use
MLH1 is a mismatch repair protein involved in maintaining the integrity of genetic information. It works alongside MSH2, MSH6, and PMS2. Mutations in the MLH1 gene can lead to defects in DNA repair pathways, which are linked to human carcinogenesis. These mutations have been reported in tumors with microsatellite instability (MSI) such as hereditary nonpolyposis colon cancer (HNPCC), some sporadic carcinomas, breast cancer, acute lymphoblastic leukemia, endometrial carcinoma, gastric carcinoma, and ovarian carcinoma.
Special Instructions
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred, or an unstained slide for H&E staining plus positively charged unstained slides for each antibody ordered. Ensure block and slide identifiers match specimen ID and labeling as noted on the requisition. Use cold pack for transport, ensuring it does not directly contact the specimen.
Limitations
This test focuses on detecting expression of MLH1 via Immunohistochemistry (IHC). It may not detect all mutations or expression variations. Correct CPT coding for billing and variations in coding practices should be confirmed with the billing payor.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Formalin-fixed paraffin-embedded tissue block or unstained slides
Storage Instructions
Use cold pack for transport, making sure it is not in direct contact with specimen.