NEO PanTracer Pro

Casandra

Casandra Test Code NG64769Version 1 (DRAFT)

Performing Lab

Lab NeoGenomicsLab Test ID N/ACPT Codes 81459, 88341, 88342, 88360, 88361

Clinical Use

Use

The pan-solid tumor assay is designed to guide therapy selection and identify clinical trial options for patients with advanced stage solid tumors. It detects genomic alterations relevant to diagnosis, therapy selection, prognosis, and clinical trial options in solid tumors. The addition of IHC and ancillary testing provides comprehensive information for therapy selection beyond standard CGP testing, ideal for patients with newly diagnosed, recurrent, or progressive advanced cancer needing comprehensive clinical investigation.

Special Instructions

Diagnosis information provided will determine tumor type-directed IHC and ancillary testing. Refer to PanTracer Pro Testing by Cancer Type for the full list of associated add-ons by cancer diagnosis.

Limitations

Only blocks from the same procedure and the same tumor should be submitted together. Use a single block for large resections where tumor is abundant, or up to 4 blocks from procedures with smaller specimens. Decalcified samples are not accepted. For HRD assessment, ≥40% tumor is required.

Test Details

New York Approved

Methodology

NGS (CGP)

Biomarkers

Detects SNVs, indels, CNVs, fusions, splice variants, and expression levels to guide therapy selection and identify clinical trial options.

Mutation

KMT2AT3, ABL1, ABL2, CDKN2A, CRLF2, CSF1R, FLT3, IKZF1, IL7R, JAK1, JAK3, NTRK1, NTRK2, NTRK3, PDGFRA, SH2B3, TP53, ASXL1, BCOR, CEBPA, CSF3R, DNMT3A, ETV6, EZH2, HRAS, IDH1, IDH2, KMT2A, NPM1, PHF6, PTPN11, RUNX1, SETBP1, SF3B1, SRSF2, STAG2, TET2, U2AF1, WT1, ZRSR2, BRIP1, DDX41, ERCC4, PALB2, RAD51C, SLX4, TERC, TERT, XRCC2, AKT1, ARID1A, ATR, BARD1, CDH1, CDK12, CTNNB1, ERBB4, FANCA, FANCC, FANCD2, FANCE, FANCF, FANCG, FANCL, GATA3, MLH1, MRE11, MSH2, MSH6, NBN, PMS2, RAD50, RAD51, RAD51B, RAD51D, RAD54L, SMAD4, SMO, SRC, APC, BAP1, PBRM1, BCL2, BIRC3, BTK, CARD11, CD79B, MYD88, NOTCH1, CXCR4, EPCAM, FBXW7, PLCG2, MUTYH, POLD1, POLE, RNF43, STK11, ATRX, ACVR1B, AKT3, AMER1, ARAF, ARFRP1, ARID1B, ARID2, AURKA, AURKB, AXIN1, AXL, BCL2L1, BCL2L2, BCL6, BCORL1, BLM, BRD4, BTG1, EMSY, CBFB, CBL, CCND2, CD274, CD79A, CDC73, CDKN1A, CDKN1B, CDKN2B, CDKN2C, CHD2, CHD4, CIC, CREBBP, CRKL, CTCF, CTNNA1, CUL3, CYLD, DAXX, DDR2, DICER1, DOT1L, EP300, EPHA3, EPHA5, EPHA7, EPHB1, ERG, ERRFI1, TENT5C, FAS, FAT1, FH, FLCN, FLT1, FLT4, FOXL2, FOXP1, FRS2, FUBP1, GABRA6, GATA1, GATA2, GATA4, GATA6, GID4, GLI1, GNA11, GNA13, GNAQ, GNAS, ADGRA2, GRIN2A, GRM3, GSK3B, HGF, H3C3, HNF1A, HSD3B1, HSP90AA1, IGF1R, IGF2, IKBKE, INHBA, INPP4B, IRF2, IRF4, IRS2, JUN, KAT6A, KDM5A, KDM5C, KDM6A, KDR, KEAP1, KEL, KLHL6, LMO1, LRP1B, LYN, LZTR1, MAGI2, MAP2K1, MAP2K2, MAP2K4, MAP3K1, MCL1, MED12, MEF2B, MEN1, MITF, MPL, MTOR, NF1, NF2, NFE2L2, NFKBIA, NKX2-1, NOTCH2, NOTCH3, NSD1, NUP93, PAK3, PAX5, PDCD1LG2, PIK3C2B, PIK3CG, PIK3R1, PIK3R2, PPP2R1A, PRDM1, PREX2, PRKAR1A, PRKCI, PRKDC, PRSS8, PTCH1, QKI, RAC1, RANBP2, RARA, RB1, RBM10, ROS1, RPTOR, RUNX1T1, SDHA, SDHB, SDHC, SDHD, SETD2, SLIT2, SMAD2, SMAD3, SMARCA4, SMARCB1, SNCAIP, SOCS1, SOX10, SOX2, SOX9, SPEN, SPOP, SPTA1, STAT3, STAT4, SUFU, SYK, TAF1, TBX3, TGFBR2, TNFAIP3, TNFRSF14, TOP1, TOP2A, TSC1, TSC2, TSHR, VEGFA, VHL, CCN6, XPO1, ZBTB2, ZNF217, ZNF703, EED, H3-3A, SUZ12, ABRAXAS1, GEN1, GREM1, HOXB13, ACVR1, ALOX12B, ANKRD11, ANKRD26, ARID5B, ASXL2, AXIN2, B2M, BBC3, BCL10, BCL2L11, BCR, BMPR1A, CALR, CASP8, CD276, CD74, CENPA, COP1, CSNK1A1, CTLA4, CUX1, DCUN1D1, DHX15, DIS3, DNAJB1, DNMT1, DNMT3B, E2F3, EGFL7, EIF1AX, EIF4A2, EIF4E, ELOC, EML4, ERCC3, ERCC5, ETS1, ETV1, ETV4, ETV5, EWSR1, FANCI, FLI1, FOXA1, FOXO1, FYN, GPS2, H1-2, H2BC5, H3-3B, H3-4, H3-5, H3C1, H3C10, H3C11, H3C12, H3C13, H3C14, H3C15, H3C4, H3C6, H3C7, H3C8, H3C2, HNRNPK, ICOSLG, ID3, IFNGR1, IGF1, IL10, INHA, INPP4A, INSR, IRS1, KIF5B, KLF4, LATS1, LATS2, MALT1, MAP3K13, MAP3K14, MAP3K4, MAPK1, MAPK3, MAX, MDC1, MGA, MSH3, MST1, MST1R, MYB, MYOD1, NAB2, NCOA3, NCOR1, NEGR1, NKX3-1, NOTCH4, NUTM1, PAK1, PAK5, PARP1, PAX3, PAX7, PAX8, PDCD1, PDPK1, PGR, PHOX2B, PIK3C2G, PIK3C3, PIK3CD, PIK3R3, PIM1, PLK2, PMAIP1, PMS1, PNRC1, PPARG, PPM1D, PPP2R2A, PPP6C, PRKN, PTPRD, PTPRS, PTPRT, RAB35, RAD21, RAD52, RASA1, RECQL4, REL, RHEB, RHOA, RIT1, RPS6KA4, RPS6KB2, RYBP, SDHAF2, SH2D1A, SHQ1, SMARCD1, SMC1A, SMC3, SOX17, STAG1, STAT5A, STAT5B, STK40, TCF3, TCF7L2, TET1, TFE3, TGFBR1, TMEM127, TMPRSS2, TP63, TRAF2, TRAF7, VTCN1, XIAP, YAP1, YES1, ZBTB7A, ZFHX3

Copy Number

MET, RET, PTEN, FGFR2, PDGFRB, MYCN, ERBB2, MYCL1, AKT2, AR, MYC, ATM, BRCA1, BRCA2, CCND1, CCND3, CCNE1, CDK4, CDK6, CHEK1, CHEK2, EGFR, ERBB3, ERCC1, ERCC2, ALK, ESR1, FGF1, FGF10, FGF14, FGF19, FGF2, FGF23, FGF3, FGF4, FGF5, FGF6, FGF7, FGF8, FGF9, FGFR1, FGFR3, FGFR4, JAK2, KIT, KRAS, LAMP1, MDM2, MDM4, NRAS, NRG1, PDGFRA, PIK3CA, PIK3CB, RAF1, RICTOR, RPS6KB1, TFRC, CDK8, BRAF

Fusion

ABL1, CSF1R, ETV6, FGFR1, FLT3, JAK2, KMT2A, NTRK1, NTRK2, NTRK3, PDGFRA, PDGFRB, ALK, BRAF, EML4, EWSR1, FGFR2, FGFR3, RAF1, ROS1, FGFR4, NOTCH1, NOTCH2, NRG1, RET, BRCA1, BRCA2, CDK4, MSH2, PIK3CA, AKT3, AXL, ERBB2, ERG, ESR1, ETS1, ETV1, ETV4, ETV5, FLI1, FLT1, KDR, KIF5B, KIT, MYC, NOTCH3, PAX3, PAX7, PPARG, RPS6KB1, TMPRSS2, KMT2AT3

MET, EGFR, ARUnknown proteinUnknown signature

Result Turnaround Time

8-10 days

Specimen

Tissue (FFPE)

Volume

Not provided

Minimum Volume

Not provided

Container

Paraffin blocks

Storage Instructions

Use cold pack for transport, ensuring cold pack is not in direct contact with specimen.

Causes for Rejection

Decalcified samples are not accepted.

Order Test

Casandra

Casandra Test Code NG64769Version 1 (DRAFT)

Performing Lab

Lab NeoGenomicsLab Test ID N/ACPT Codes 81459, 88341, 88342, 88360, 88361