NPM1 Mutation Analysis
Also known as: Nucleophosmin (Nucleolar Phosphoprotein B23)
Use
Testing for NPM1 and other gene mutations in AML patients with intermediate-risk cytogenetic abnormalities can improve risk stratification. NPM1 mutations can predict favorable prognosis in AML with normal karyotype.
Special Instructions
A paraffin block is preferred for FFPE. Do not use zinc fixatives. Use positively-charged slides and 10% NBF fixative. Ensure block and slide identifiers match the specimen ID and labeling as noted on the requisition.
Limitations
The test is optimized for detecting small insertion mutations specific to AML in exon 12 of NPM1. The process requires precise handling of specimens and may not be suitable for samples with incompatible fixatives.
Methodology
PCR-based (PCR)
Biomarkers
Result Turnaround Time
7 days
Related Documents
For more information, please review the documents below
Specimen
Bone Marrow
Volume
2 mL
Minimum Volume
Not provided
Container
EDTA tube
Storage Instructions
Use cold pack for transport. Refrigerate before shipping and ship same day as drawn whenever possible. Do not freeze.