NUT Immunohistochemistry (IHC)
Use
Expression of nuclear protein in testis (NUT) is generally confined to the germ cells of the testis and ovary. NUT midline carcinomas are aggressive tumors with non-diagnostic morphology that overlaps with many other poorly differentiated tumors, but are characterized by rearrangement of the NUTM1 (NUT) gene at chromosome 15q14 with BRD4/3 (and rarely NSD3, ZNF532 or ZNF592), which causes NUT protein overexpression.
Special Instructions
IHC staining with NUT antibody may serve as a diagnostic alternative to FISH or molecular confirmation of 15q14 rearrangement.
Limitations
NUT overexpression by IHC may also be seen in other diseases with NUTM1 rearrangement, such as porocarcinoma with YAP1-NUTM1 gene fusion. Therefore, a diagnosis of NUT midline carcinoma should not be based on NUT IHC positivity alone.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
24 hours
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Block and slide identifiers should be clearly written and match exactly with the specimen ID and specimen labeling as noted on the requisition.
Storage Instructions
Use cold pack for transport, making sure cold pack is not in direct contact with specimen.