pHistone H3 (PHH3)
Use
Phosphohistone H3 (PHH3) is a marker of cells in the late G2-M phase of the cell cycle. It is not expressed in apoptotic cells which may be confused with mitotic figures on a routine H&E stained slide. PHH3 can be used as a surrogate of mitotic activity or as an independent prognostic marker in breast carcinomas.
Special Instructions
Not provided.
Limitations
Not provided.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
formalin-fixed, paraffin-embedded (FFPE) tissue block or unbaked, unstained slide
Collection Instructions
A formalin-fixed, paraffin-embedded (FFPE) tissue block is preferred or one (1) unbaked, unstained slide for H&E staining (required) and two to three (2-3) positively charged unstained slides (cut at 4-5 microns) for each test/antibody ordered.
Storage Instructions
Use cold pack for transport, make sure cold pack is not in direct contact with specimen.
Causes for Rejection
Block and slide identifiers not matching specimen ID and specimen labeling as noted on the requisition.