Live cell-based assay to clustered acetylcholine receptor antibodies (AChR CBA)
Use
This test assists in the diagnosis of autoimmune myasthenia gravis (MG) by detecting acetylcholine receptor (AChR) antibodies. It provides more sensitive detection of AChR antibodies, particularly in double-seronegative samples that may remain undetected by conventional methods like RIPA or ELISA.
Special Instructions
Not provided.
Limitations
Causal antibodies cannot be identified in about 10% of MG cases. Therefore, a positive result is specific for AChR Ab myasthenia gravis but a negative result does not rule out an MG diagnosis. The test has not been cleared by the FDA.
Methodology
Cell-based / Cytometry
Biomarkers
Acetylcholine receptor antibodies
Protein
Result Turnaround Time
2-7 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
3 mL
Minimum Volume
0.5 mL
Container
Gel barrier or red-top tube
Collection Instructions
No patient preparation is required. Use SST activator tubes, invert 8 times, allow sample to clot, then centrifuge. Draw off serum using a pipette into aliquot tubes.
Storage Instructions
Store aliquot tubes in refrigerator (2° C - 8° C) until shipping.
Causes for Rejection
Grossly hemolytic, lipemic, or icteric samples; samples arriving at room temperature.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 2 weeks |
| Frozen | 5 months |