Amikacin, Peak
Also known as: Amikin
Use
Amikacin is an aminoglycoside antibiotic used in the treatment of serious gram‑negative infections. Due to its narrow therapeutic window and potential toxicity, monitoring serum/plasma concentrations of amikacin—particularly peak levels—is clinically important to ensure therapeutic effectiveness while minimizing risk of nephro- or ototoxicity.
Special Instructions
Collect a blood specimen approximately 30 minutes post‑infusion to measure the peak level accurately. Process by centrifuging and aliquoting serum or plasma; if there will be a delay in analysis of more than 8 hours, sample should be frozen. Transport aliquoted specimen frozen to laboratory.
Limitations
Falsely low amikacin levels may result if samples containing additional antibiotics are not frozen promptly. Delayed processing or improper storage can affect accuracy of the result. This assay is peak‑level specific, and results may not reflect trough or random levels unless those are ordered separately.
Methodology
Immunoassay (ELISA)
Biomarkers
Result Turnaround Time
Not provided.
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL (serum) or 500 µL (microtainer)
Minimum Volume
Not provided
Container
Serum red‑top, serum separator tube or microtainer; plasma EDTA, heparin, or fluoride/oxalate tube
Collection Instructions
Collect 30 minutes post infusion of IV dose for peak level.
Storage Instructions
Freeze if delay in analysis more than 8 hours; transport frozen.