BK Virus DNA, Quantitative, Real‑Time PCR, Urine
Also known as: BK Virus DNA, QT Real-Time PCR, Polyomavirus, BK Viral Load, BKV Polyoma
Use
This test detects and quantitates BK Virus DNA in urine specimens of kidney transplant recipients who are at risk of developing BKV‑associated nephropathy (BKVAN) and associated hemorrhagic cystitis leading to kidney failure. Prospective viral load monitoring of BKV DNA in urine is useful to monitor the course of BKV infection and exclude a diagnosis of BKVAN. The assay is calibrated to the WHO International Standard for BKV DNA, with a reportable range of 200–100,000,000 IU/mL (2.30–8.00 log IU/mL).
Special Instructions
Neat urine must be immediately stabilized by transferring to a cobas® PCR Media tube; liquid must fall between the black fill lines on the tube. Specimen must be transported refrigerated. Only urine submitted in cobas® PCR Media tube is acceptable; neat urine is unacceptable.
Limitations
Not provided.
FDA Approved
Methodology
PCR-based (PCR)
Biomarkers
LOINC Codes
- 32285-9
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Urine
Volume
4.3 mL
Minimum Volume
4.3 mL
Container
cobas® PCR Media tube
Collection Instructions
Neat urine must be immediately stabilized by transferring to a cobas® PCR Media tube; liquid level between the black fill lines
Causes for Rejection
Under‑ or over‑filled cobas PCR urine sample tubes; neat urine
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 90 days |
| Refrigerated | 90 days |
| Frozen | Unacceptable |