CD16A (FCGR3A) F158V Polymorphism Analysis
Also known as: Fc engineered monoclonal antibody, 158 F allele, V176F, Missense mutation, Phe158Val
Use
The CD16A (FCGR3A) gene encodes an Fc‑gamma receptor on human white blood cells, playing a critical role in antibody‑dependent cell‑mediated cytotoxicity (ADCC). In cancer patients, differential clinical responses to therapeutic monoclonal antibodies have been correlated with CD16A (FCGR3A) genotypes (V/V, F/V, or F/F) at SNP rs396991 (T>G, resulting in phenylalanine to valine at position 158) (PMID: 23286345; 30374078; 33480963).
Special Instructions
Not provided.
Limitations
Not provided.
Methodology
PCR-based (PCR)
Biomarkers
FCGR3A
Gene
Result Turnaround Time
6 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL
Minimum Volume
3 mL whole blood
Container
EDTA (lavender‑top) tube, or ACD A/B (yellow‑top) tube
Causes for Rejection
Samples received frozen or clotted
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Whole blood: 8 days |
| Refrigerated | 30 days |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant