Cholinesterase, RBC and Plasma
Also known as: RBC Cholinesterase
Use
True cholinesterase (RBC and plasma) activity is decreased in individuals with exposure to organophosphorous insecticides. True cholinesterase, found in erythrocytes and nerve tissue, is responsible for inactivating acetylcholinesterase at nerve endings. With decreased enzyme activity, patients may display a range of nervous system dysfunction. Analysis of RBC and serum or plasma activity is useful in monitoring exposure and recovery.
Special Instructions
Not provided.
Limitations
Elevated RBC values, caused by the presence of young RBCs and reticulocytes, may mask acetylcholinesterase inhibition. Plasma results may be depressed in patients who consume oral contraceptives. Hepatic disease may cause up to a 70% decrease in enzyme levels. Cholinesterase may be slightly elevated in patients with obesity or diabetes.
Methodology
Other
Biomarkers
Result Turnaround Time
Not provided.
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL (4 mL minimum)
Minimum Volume
4 mL
Container
EDTA (lavender-top) tube
Collection Instructions
Draw two EDTA (lavender‑top) tubes of whole blood. Spin one tube to separate plasma. Pour plasma into plastic aliquot tube. Send one 5 mL refrigerated uncentrifuged whole blood (4 mL minimum) and 1 mL refrigerated plasma (0.5 mL minimum). Do not send packed cells. Do not send only one tube of whole blood.
Storage Instructions
Refrigerate (cold packs)
Causes for Rejection
Hemolysis • Lipemia • Received room temperature • Receipt of only a single tube of whole blood
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 7 days |
| Frozen | Unacceptable |