Factor VIII Inhibitor Panel
Also known as: FVIII Bethesda Inhibitor, Factor VIII Inactivity, Inhibitor APTT, Factor 8 Inhibitor Evaluation
Use
The Factor VIII Inhibitor Panel (test code 40083) is used to detect and quantify inhibitors (alloantibodies or autoantibodies) against factor VIII, which may occur in patients with congenital hemophilia A undergoing replacement therapy or in acquired hemophilia. Low factor VIII activity triggers reflex testing: a chromogenic IgG antibody screen and, if positive, quantification by the Nijmegen‑Bethesda assay to determine inhibitor titer in Bethesda Units (BU), reflecting the functional inhibitory activity.
Special Instructions
Not provided.
Limitations
Not provided.
Methodology
Immunoassay (ELISA)
Biomarkers
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
two–three 2 mL platelet‑poor plasma
Minimum Volume
two–three 1 mL platelet‑poor plasma
Container
Light Blue (Sodium Citrate) tube
Collection Instructions
Collect three–four 1.8 mL or two–three 2.7 mL blood in light blue‑top sodium citrate tube; centrifuge to obtain platelet‑poor plasma (free of platelets <10,000/mcL), freeze immediately; plasma must be frozen for transport.
Storage Instructions
Freeze immediately and send frozen
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Unacceptable |
| Refrigerated | Unacceptable |
| Frozen | 14 Days |