FISH, CML/ALL, bcr/abl Translocation 9;22
Also known as: CML, ALL, Philadelphia Chromosome, Chronic Myelogenous Leukemia (CML), Acute Lymphoblastic Leukemia (ALL)
Use
This test uses fluorescence in situ hybridization (FISH) to detect molecular rearrangement of the BCR and ABL1 genes involved in t(9;22). The Philadelphia chromosome (Ph) resulting from this translocation is associated with chronic myelogenous leukemia (CML), de novo acute myeloid leukemia (AML), and acute lymphocytic leukemia (ALL). It may also be used in evaluation of Ph‑negative CML and complex rearrangements.
Special Instructions
Clinical history and reason for referral are required with test order. Prior therapy and bone marrow transplant history should be provided. Insurance authorization may be required for outpatients; inpatients may be okay to send without prior authorization.
Limitations
Specimen viability decreases during transit. Specimens must not be frozen; if not viable, the test order may be canceled and replaced by a "Cytogenetics Communication" order.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
5 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5 mL whole blood
Minimum Volume
3 mL whole blood
Container
Sodium heparin (green‑top) tube
Collection Instructions
Preferred 5 mL whole blood in sodium heparin green‑top tube
Storage Instructions
Room temperature
Causes for Rejection
Do not freeze