Use

Translocations involving the immunoglobulin loci are recurring events in various subtypes of B‑cell lymphomas. In addition to translocations involving the IGH locus, variant translocations have been described in 5‑10% of B‑cell neoplasms involving either the immunoglobulin kappa (IGK) light chain or the lambda light chain (IGL). The best known translocations involving IG light chain loci are the variant Burkitt's translocations t(2;8)(p12;q24) and t(8;22)(q24;q11), present in up to 25% of all Burkitt's lymphomas. Other translocations involve the BCL6 oncogene (t(2;3)(p12;q27) and t(3;22)(q27;q11)) and BCL2 locus (t(2;18)(p12;q21) and t(18;22)(q21;q11)) in follicular lymphoma. These IG light chain translocations may occur in combination in 'double hit' and 'triple hit' lymphomas. The IGK break‑apart FISH probe allows for definitive identification of IGK rearrangement at 2p11.2 in both suspension cultures and FFPE tissues, facilitating clarification of complex karyotypes.