FISH, Mantle Cell Lymphoma, IGH‑CCND1, t(11;14)

Casandra

Casandra Test Code QD24563Version 1 (DRAFT)

Performing Lab

Lab Quest DiagnosticsLab Test ID 17346

FISH, Mantle Cell Lymphoma, IGH‑CCND1, t(11;14)

Clinical Use

Use

Detection of the t(11;14)(q13;q32) translocation involving the IGH (immunoglobulin heavy chain) locus on chromosome 14 and CCND1 (cyclin D1, BCL1) on chromosome 11, characteristic of mantle cell lymphoma and found variably in other B‑cell neoplasms including B‑prolymphocytic leukemia, plasma cell leukemia, chronic lymphocytic leukemia, and multiple myeloma. This translocation results in cyclin D1 overexpression and is used to detect abnormal clones for diagnosis, prognosis, and response to therapy monitoring. ([saintfrancis.com](https://www.saintfrancis.com/physicians/laboratory-testing/cytogenetics/available-tests/fish-analysis-ccnd1-igh-translocation?utm_source=openai))

Special Instructions

Specimen requirements include bone marrow aspirate (2 mL, sodium heparin green‑top) or peripheral blood (5 mL in sodium heparin). Minimum volumes are 1 mL bone marrow or 2 mL blood. Specimens must arrive at ambient temperature (20‑25 °C) within 24 hours; refrigerate if delayed. Frozen, clotted specimens, paraffin‑embedded tissue, or specimens in other anticoagulants are unacceptable. A cytogenetics/FISH requisition must accompany the specimen. ([saintfrancis.com](https://www.saintfrancis.com/physicians/laboratory-testing/cytogenetics/available-tests/fish-analysis-ccnd1-igh-translocation?utm_source=openai))

Limitations

FISH analysis may be limited by low clone frequency or poor specimen quality. Detection of t(11;14) indicates presence of abnormal clone but a negative result does not exclude neoplastic disease. Cultured or enriched plasma cells may lose viability; delays in specimen transit may compromise results. ([saintfrancis.com](https://www.saintfrancis.com/physicians/laboratory-testing/cytogenetics/available-tests/fish-analysis-ccnd1-igh-translocation?utm_source=openai))

Test Details

Methodology

Chromosomal / Cytogenetics (FISH)

Biomarkers

Dual‑color, dual‑fusion fluorescence in situ hybridization analysis of IGH locus (14q32) and CCND1 (11q13) on interphase cells to detect the t(11;14) translocation signature.

Unknown CNV

Copy Number Region

FISH IGH‑CCND1 dual‑fusion • Copy Number • Categorical (e.g., Positive / Negative / Indeterminate)

Unknown CNV

Copy Number Region

FISH IGH‑CCND1 dual‑fusion • Copy Number • Categorical (e.g., Positive / Negative / Indeterminate)