HBME-1, IHC without Interpretation
Use
HBME‑1 (antibody) immunohistochemistry (IHC) serves as a marker to help identify mesothelial cells. It is frequently used in conjunction with other markers, such as CK19, to assist in differentiating papillary thyroid carcinoma from benign lesions. While sensitive for papillary thyroid cancer, diffuse and intense membranous staining is most characteristic, though cross‑reactivity with other tumors (e.g., ovarian, lung, gastrointestinal origin) may occur.
Special Instructions
Not provided.
Limitations
Fixatives other than 10% neutral buffered formalin may yield suboptimal or non‑equivalent staining results. Interpretation of HBME‑1 staining must consider potential cross‑reactivity with tumors of non‑thyroid origin, and staining patterns should be interpreted in the context of histomorphology and additional immunohistochemical markers.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
0.2 cm × 0.2 cm × 0.2 cm tissue; minimum of 2 unstained slides per antibody in 5 µm sections
Minimum Volume
Not provided
Collection Instructions
Formalin‑fixed, paraffin‑embedded (FFPE) tissue block and/or unstained slides (5 µm sections). Precut slides acceptable; mount on positively charged slides. Do not oven dry unstained slides; air dry. Send unstained slides sectioned from tissue block.
Storage Instructions
Store at room temperature; ambient shipment acceptable; do not freeze.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |