Hemoglobin, Free, Plasma
Use
The presence of free hemoglobin in plasma is an indication of intravascular hemolysis resulting from numerous conditions, including Paroxysmal Nocturnal Hemoglobinuria (PNH), Sickle‑Cell Disease (SCD), Thalassemias, Hereditary Spherocytosis, Microangiopathic Hemolytic Anemias, Pyruvate Kinase Deficiency, ABO mismatch transfusion reaction, cardiopulmonary bypass, or mechanical heart valve‑induced anemia.
Special Instructions
Specimen should be collected as plasma in a sodium heparin (green‑top) tube; centrifuge plasma within 1 hour of collection, then transfer plasma to a sterile plastic screw‑cap vial for transport. Alternate acceptable specimen: lithium heparin tube. Do not freeze.
Limitations
High bilirubin (>20 mg/dL), turbidity, methemalbuminemia, lipemic plasma, and hemolysis during or after venipuncture may cause falsely elevated values in this plasma hemoglobin test.
New York Approved
Methodology
Other
Biomarkers
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1 mL preferred; minimum 0.2 mL
Minimum Volume
0.2 mL
Container
Plastic screw‑cap vial
Collection Instructions
Collect plasma in sodium heparin (green‑top) tube; centrifuge within 1 hour; transfer to sterile plastic screw‑cap vial.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 7 days |
| Frozen | Unacceptable |