HIV‑1 RNA, Quantitative, Real‑Time PCR, CSF
Use
Quantitative detection of HIV‑1 RNA in cerebrospinal fluid (CSF) provides a sensitive assessment of central nervous system (CNS) viral burden, useful in evaluating CNS involvement, diagnosing HIV-associated neurologic conditions, and monitoring treatment efficacy within the CNS compartment.
Special Instructions
Not provided.
Limitations
Heparin inhibits PCR and must not be used; CSF is the only acceptable specimen type; performance characteristics validated under CLIA but not FDA–cleared; test may be referred out and turnaround depends on transport and processing delays.
Methodology
PCR-based (RT-PCR)
Biomarkers
HIV‑1 RNA
Analyte
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
3.0 mL
Minimum Volume
1.1 mL
Container
Sterile screw‑capped container
Collection Instructions
Collect at least 3 mL CSF in a sterile screw‑capped container; do not use heparin tube; aliquot into red‑top sterile transport tube; ship CSF frozen.
Causes for Rejection
Heparin tube collection
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 6 days |
| Frozen | 42 days |
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