Mycoplasma pneumoniae DNA, Qualitative, Real-Time PCR
Also known as: M. pneumoniae by PCR
Use
This assay employs highly sensitive real‑time PCR to detect Mycoplasma pneumoniae DNA in clinical specimens. A positive result should be interpreted within the clinical context as PCR may detect non‑viable organisms, and a negative result does not fully exclude infection at undetectable levels.
Special Instructions
Not provided.
Limitations
This PCR‑based assay cannot distinguish viable from non‑viable Mycoplasma pneumoniae organisms. Negative results indicate DNA was not detected at measurable levels but do not definitively rule out infection.
Methodology
PCR-based (RT-PCR)
Biomarkers
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Sputum
Volume
1 mL
Minimum Volume
0.35 mL
Container
sterile, leak‑proof plastic container
Collection Instructions
Collect in sputum collection kit or sterile, leak‑proof plastic container.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 7 days |
| Frozen | 30 days |