Progesterone Receptor (PR) IHC with Interpretation
Use
Immunohistochemical detection of progesterone receptor (PR) expression serves as an important prognostic and predictive marker in breast carcinoma and potentially other hormonally responsive tumors. This information aids clinicians in assessing tumor biology and tailoring endocrine therapy strategies.
Special Instructions
Submit formalin‑fixed, paraffin‑embedded (FFPE) tissue; ensure proper fixation per ASCO/CAP guidelines (typically 10% neutral buffered formalin for 6–72 hours). Slides should be on charged (Plus) glass and unstained slides or blocks may be required. Interpretive reporting is provided via pathologist IHC interpretation.
Limitations
Fixatives other than 10% formalin may adversely affect performance. Decalcified or inadequately fixed tissue may yield unreliable results. Pre‑analytical variables like ischemic time or fixation duration outside validated windows can limit interpretive accuracy.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
FFPE tissue block or unstained slides (5‑micron sections), minimum of 2 slides; mounted on Poly‑L‑Lysine‑coated or Plus (+) slides.
Storage Instructions
Store at room temperature; unstained slides should be sent within 6 weeks of cutting.