Trichinella Antibody (IgG), ELISA
Use
This test serves as an adjunct in the diagnosis of trichinosis, aiding in detection of IgG antibodies to Trichinella spiralis; results must be interpreted alongside clinical presentation, epidemiologic context, and other laboratory findings. A positive ELISA result suggests current or recent infection; however, seroconversion may not occur until approximately three weeks post–infection, so negative results in suspicious cases should prompt repeat testing in a few weeks.
Special Instructions
This is performed as a send‑out test to Quest Diagnostics (Nichols Institute, San Juan Capistrano, CA) under Mayo Clinic send‑out code ZW131 with referral lab code 34321. Collection requires blood drawn into gold SST or red‑top tube, centrifuged, and serum frozen. Handling and transport procedures must follow Quest's guidance.
Limitations
Antibodies may not be detectable until approximately three weeks after infection; cross‑reactivity with other parasitic antigens (e.g., Strongyloides, filaria, malaria) may occur, particularly causing equivocal results. Antibody may remain detectable for two to three years following infection and treatment, limiting differentiation of past versus recent exposure.
Methodology
Immunoassay (ELISA)
Biomarkers
Result Turnaround Time
1-7 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.1 mL
Collection Instructions
Collect blood in gold SST or red‑top tube; centrifuge and transfer serum into plastic vial; freeze serum at –20 °C while awaiting shipment.
Causes for Rejection
Gross hemolysis, gross lipemia, heat‑inactivated specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 14 days |
| Frozen | 30 days |