Tryptase
Use
Tryptase is used for evaluating mast cell activation events such as anaphylaxis or allergen challenges and conditions like systemic mastocytosis or mast cell activation syndrome. Its levels in serum reflect the extent of mast cell degranulation, as tryptase is predominantly released from mast cell granules after activation. Basophils also contribute small amounts. Elevated serum tryptase is clinically relevant for diagnosing mast cell–mediated reactions.
Special Instructions
Blood should be drawn between 15 minutes and 3 hours after suspected mast cell activation. Samples must be separated promptly (within 3 hours of collection). Refrigerated or frozen specimens are acceptable for transport. Collection details indicate importance of timing relative to clinical events.
Limitations
Not provided.
Methodology
Immunoassay
Biomarkers
Result Turnaround Time
4-8 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1-2 mL Blood (to yield ~1 mL serum)
Minimum Volume
0.5 mL Serum
Container
Red Top (No Additive)
Collection Instructions
Samples should be obtained between 15 minutes and 3 hours after the event suspected of causing mast cell activation; separate serum within 3 hours of collection.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 Days |
| Frozen | 30 Days |