Acanthamoeba and Naegleria Culture and Stain, CSF
Also known as: ACANT CSF
Use
This test is used to detect the presence of free-living amoebae such as Acanthamoeba species and Naegleria fowleri in cerebrospinal fluid (CSF) specimens. The culture method identifies the presence of Acanthamoeba species and Naegleria fowleri, while the stain detects additional free-living amoebae such as Balamuthia mandrillaris, which the culture method cannot detect. This test is significant for diagnosing amoebic infections, which can lead to severe, often fatal conditions if untreated.
Special Instructions
For ordering this test, a CSF specimen must be collected and transferred to a sterile ARUP Standard Transport Tube. The tube can be ordered through ARUP Connect or by contacting Client Services. It is important to follow proper specimen preparation and collection techniques to ensure test accuracy.
Limitations
The culture method used in this test will not detect Balamuthia mandrillaris, as it is specifically designed for Acanthamoeba species and Naegleria fowleri. Further, specimens in media or preservatives are unacceptable for this test. Additionally, the test's stability window at ambient temperature is limited to 72 hours, and specimens stored refrigerated or frozen are not acceptable.
New York Approved
Methodology
Culture-based
Biomarkers
LOINC Codes
- 9781-6
- 87965-0
Result Turnaround Time
2-10 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
1 mL
Minimum Volume
0.5 mL
Container
sterile ARUP Standard Transport Tube
Causes for Rejection
Specimens in media or preservatives.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | Unacceptable |
| Frozen | Unacceptable |
Other tests from different labs that may be relevant