Acute Myeloid Leukemia (AML), Children's Oncology Group Enrollment Testing, FISH, Varies
Use
This test is crucial for detecting recurrent common chromosome abnormalities associated with acute myeloid leukemia (AML) in patients being considered for enrollment in Children's Oncology Group (COG) clinical trials and research protocols. It serves as an adjunct to conventional chromosome studies in pediatric patients with AML and is particularly valuable for evaluating specimens in which chromosome studies are unsuccessful. By pinpointing specific chromosomal abnormalities, the test aids in the diagnostic categorization and treatment planning for pediatric AML patients. However, it should not be used to screen for residual AML.
Special Instructions
This test is performed as panel testing specifically for pediatric patients being considered for enrollment in a Children's Oncology Group (COG) protocol. Additional charges will apply for reflex or additional probe sets and are based on the number of cells analyzed per probe set. It is necessary to provide the COG registration and protocol number with each specimen, though testing will not be rejected if omitted. Test results will be reported following the analysis of interphase nuclei using FISH probes designed to detect specific chromosomal abnormalities.
Limitations
While the test offers vital diagnostic insights into chromosomal abnormalities in AML, it is not approved by the FDA and is best used in conjunction with conventional chromosome studies and comprehensive clinical evaluation. FISH analysis is not a replacement for conventional chromosome studies and may miss abnormalities detectable through broader cytogenetic evaluations. The preferred specimen type is bone marrow; blood specimens may be used if they contain circulating malignant cells, as verified by a hematopathologist. Furthermore, no FISH signals observed post-hybridization will result in results indicating a lack of FISH findings.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
LOINC Codes
- 102101-3
- 50397-9
- 69965-2
- 93356-4
- 62356-1
- 42349-1
- 31208-2
- 85069-3
- 48767-8
- 62364-5
- 18771-6
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Bone Marrow
Volume
2 to 3 mL
Minimum Volume
1 mL
Container
Yellow top (ACD)
Collection Instructions
Send bone marrow specimen in original tube. Do not aliquot. It is preferable to send the first aspirate from the bone marrow collection. Invert several times to mix bone marrow.
Causes for Rejection
Fresh tissue