Anti-Neutrophil Cytoplasmic Antibody, IgG by IFA
Also known as: ANCA-IFA
Use
Neutrophil Cytoplasmic Antibodies (C-ANCA = granular cytoplasmic staining, P-ANCA = perinuclear staining) are found in the serum of over 90 percent of patients with certain necrotizing systemic vasculitides, and usually in less than 5 percent of patients with collagen vascular disease or arthritis. Detection of ANCA should be followed by testing for MPO-ANCA/PR3-ANCA to determine antibody specificity; ANCA may be positive in the absence of PR3-ANCA and MPO-ANCA in a number of other systemic or inflammatory diseases. This test is not recommended as a first-line screening test for ANCA-associated vasculitis.
Special Instructions
ANCA IFA is simultaneously tested on ethanol- and formalin-fixed slides to allow differentiation of C- and P-ANCA patterns.
Limitations
ANCA may be positive in the absence of PR3-ANCA and MPO-ANCA, complicating interpretation in the context of other systemic or inflammatory diseases. Additionally, this test is not recommended as a first-line screening test for ANCA-associated vasculitis. False positives may occur in infections and other autoimmune conditions.
New York Approved
Methodology
Immunoassay (IFA)
Biomarkers
LOINC Codes
- 21419-7
- 29967-7
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.15 mL
Container
ARUP standard transport tube
Collection Instructions
Separate serum from cells ASAP or within 2 hours of collection.
Storage Instructions
Refrigerated.
Causes for Rejection
Plasma, urine, or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 2 weeks |
| Frozen | 30 days (avoid repeated freeze/thaw cycles) |
Other tests from different labs that may be relevant