Antichromatin Antibodies
Also known as: Antibodies to LE Cell Phenomenon
Use
Help to diagnose drug-induced lupus (DIL) and systemic lupus erythematosus (SLE). Antibodies to both chromatin and histones have been found in patients with procainamide-induced lupus; however, patients with lupus induced by drugs such as quinidine, penicillamine, methyldopa, and acebutolol have antibodies to chromatin but not antihistone. Antichromatin antibodies are found in 50% to 90% of SLE patients and have been linked with proteinuria in SLE patients.
Special Instructions
If using a tube other than a gel-barrier tube, ensure separated serum is transferred to a plastic transport tube to maintain sample integrity.
Limitations
The presence of antichromatin antibodies, while indicative of certain conditions, does not confirm a diagnosis on its own. Results should be considered in the context of other clinical findings. Hemolysis of samples can lead to rejection. Room temperature, refrigerated, or frozen storage should each not exceed 14 days, and freeze/thaw cycles should be limited to three to prevent sample degradation.
Methodology
Immunoassay (Multiplex Flow Immunoassay)
Biomarkers
LOINC Codes
- 51775-5
- 51775-5
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL
Container
Gel-barrier tube or red-top tube
Collection Instructions
If tube other than gel-barrier tube is used, transfer the separated serum to a plastic transport tube.
Storage Instructions
Room temperature storage: up to 14 days. Refrigerated storage: up to 14 days. Frozen storage: up to 14 days. Limit freeze/thaw cycles to three.
Causes for Rejection
Hemolysis
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |
Other tests from different labs that may be relevant