B-cell lg Heavy Chain Gene Rearrangement
Use
This assay is used to detect clonal IGH rearrangements to assist in diagnosis of B-cell lymphoproliferative disorders. It is crucial for identifying clonality in leukemia and lymphoma, which are B-cell related cancers, and aids in differentiating between reactive and neoplastic conditions.
Special Instructions
Frozen bone marrow samples are NOT accepted. Paraffin Embedded Tissue Block: FFPE block preferred; unbaked slides acceptable. Extracted DNA: Isolation of nucleic acids for clinical testing must occur in a CLIA-certified laboratory or a laboratory meeting equivalent requirements. Concentration and volume must be provided for all samples.
Limitations
The assay's performance may be affected by the quality and quantity of the DNA extracted. Use of non-preferred specimens might lead to suboptimal results. Improper specimen labeling and insufficient sample volume can lead to rejection. Results are specifically applicable to B-cell clonality and not other conditions outside the context of B-cell lymphoproliferative disorders.
Methodology
NGS (Targeted)
Biomarkers
Result Turnaround Time
14 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
5mL
Minimum Volume
3mL
Container
EDTA preferred (NaHep accepted)
Storage Instructions
2°C to 8°C
Causes for Rejection
Improper specimen labeling; Insufficient sample volume; Clotted specimen; Specimens beyond stability
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinite |
| Refrigerated | 30 Days |
| Frozen | Long Term |