BCL2
Use
BCL2 is a member of a family of proteins involved in apoptosis with wide tissue distribution. In 90% of follicular lymphomas, a translocation occurs which juxtaposes the BCL2 gene at 18q21 to an immunoglobulin gene. This t(14;18) translocation can deregulate gene expression and results in BCL2 overexpression, which can be detected via IHC.
Special Instructions
Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. This is NOT preferred. Samples must be tested 3 months after placing in ethanol. Unstained slides: Positively charged glass slides are required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected. Fresh tissue should be at a 20:1 fixative to tissue ratio, with 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours preferred).
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Fresh Tissues: AMBIENT, use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |