BCL6 (3q27) Gene Rearrangement by FISH

Casandra

Casandra Test Code AR63911Version 1 (DRAFT)

Performing Lab

Lab ARUP LaboratoriesLab Test ID 3001311CPT Code 88366

BCL6 (3q27) Gene Rearrangement by FISH

Clinical Use

Use

This test is used for detecting BCL6 gene rearrangements in formalin-fixed, paraffin-embedded (FFPE) tissues, particularly in cases of B-cell lymphomas such as diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma. The presence of BCL6 rearrangements can be crucial for prognosis and diagnosis in these lymphomas. It helps in correlating clinical, morphological, and immunophenotypic data to improve patient management.

Special Instructions

Include the surgical pathology report with the specimen submission. If submitting multiple specimens, include an order comment specifying specimen selection by pathologist or submit individual orders for each sample. A pathologist block selection fee is applicable if asking ARUP to choose the specimen for testing.

Limitations

This FISH test is designed to detect translocations involving the 3q27 locus (BCL6) without regard to partner chromosomes. It is sensitive to BCL6 rearrangements, except in rare cryptic rearrangements. Results should be interpreted within the context of clinical and pathological information. The test’s performance was validated with known positive and normal cases, but it is not cleared by the FDA and should be used for clinical purposes only.

Test Details

New York Approved

Methodology

Chromosomal / Cytogenetics (FISH)

Biomarkers

Unknown CNV

Copy Number Region

Copy Number Alteration • Categorical (e.g., Positive / Negative / Indeterminate)

LOINC Codes

Result Codes

21746-3

Result Turnaround Time

3-7 days

Specimen

Tumor Tissue (FFPE)

Volume

Not provided

Minimum Volume

2 slides

Container

Tissue transport kit (ARUP supply #47808 recommended but not required)

Collection Instructions

Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen. Transport tissue block or 5 unstained (3-micron thick sections), positively charged slides. Do not oven bake pre-cut slides.

Storage Instructions

Protect paraffin block and/or slides from excessive heat. Ship in cooled container during summer months.

Causes for Rejection

Paraffin block with no tumor tissue remaining. Specimens fixed in any fixative other than 10 percent neutral buffered formalin. Decalcified specimens.