Bordetella pertussis and Bordetella parapertussis, Molecular Detection, PCR, Varies
Use
This test is primarily used for the detection of Bordetella pertussis and Bordetella parapertussis. It is preferred in diagnostic settings to detect these pathogens, which are the causes of pertussis or whooping cough, a highly contagious respiratory disease. Bordetella parapertussis causes a less severe illness, but detection of both is critical due to the limitations of vaccination in preventing Bordetella parapertussis infection. Accurate detection is vital for effective disease control, especially as pertussis remains common despite vaccination efforts.
Special Instructions
Specimen source information is required for submission, and all specimens should be processed in a contamination-free environment to ensure the sensitivity of PCR amplification is preserved. Various specimens like nasopharyngeal swab, nasal aspirate, and wash are acceptable, but note specific collection and transport requirements to avoid rejection.
Limitations
The PCR assay may cross-react with Bordetella holmesii and Bordetella bronchiseptica due to shared insertion sequences, which can potentially lead to false positives. The presence of PCR-detectable DNA in some acellular vaccines can also cause false-positive results if samples are contaminated. Sensitivity may be reduced over time post-symptom onset due to decreased DNA presence, and serological testing can supplement PCR for later stages of infection.
New York Approved
Methodology
PCR-based
Biomarkers
LOINC Codes
- 90441-7
- 31208-2
- 43913-3
- 42588-4
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Nasopharyngeal Swab
Volume
Not provided
Minimum Volume
0.5 mL
Container
Rayon swab with aluminum shaft in transport medium
Collection Instructions
Nasopharyngeal swab should be placed in a transport medium such as Stuart's media with or without charcoal, or Amies media with or without charcoal.
Storage Instructions
Refrigerated preferred. Stable for up to 7 days at refrigerated, ambient, or frozen temperatures.
Causes for Rejection
Swab transported in gel medium, dry swab, viral/universal transport medium, Regan Lowe media, and other media types that may inhibit PCR are rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 7 days |
| Frozen | 7 days |