Borrelia miyamotoi Detection, PCR, Blood
Use
Aiding in the diagnosis of Borrelia miyamotoi infection in conjunction with clinical findings, this test is the preferred method for detection of B. miyamotoi using blood specimens. Borrelia miyamotoi is a spirochetal bacterium causing a febrile illness similar to tick-borne relapsing fever, with symptoms like body and joint pain, fatigue, and occasionally rash. The organism is detected in Ixodes scapularis and Ixodes pacificus ticks, which are also vectors for Lyme disease, anaplasmosis, and babesiosis.
Special Instructions
This assay does not detect the Borrelia species that cause Lyme disease. If Lyme disease is suspected, order the Lyme Antibody Modified 2-Tier with Reflex, Serum test. If not ordering electronically, submit a Microbiology Test Request Form (T244) with the specimen.
Limitations
Inadequate specimen collection or improper storage may invalidate test results. After adequate treatment, Borrelia miyamotoi DNA may remain detectable for an unknown period. The assay is designed to detect only species of clinical significance and should not be used to screen healthy patients. It does not differentiate subspecies or strains of Borrelia miyamotoi.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 82475-5
- 82475-5
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
1 mL
Minimum Volume
0.3 mL
Container
Lavender top (EDTA); Acceptable: Royal blue top (EDTA), pink top (EDTA), or sterile vial containing EDTA-derived aliquot
Collection Instructions
Send whole blood specimen in original tube (preferred).
Causes for Rejection
Gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |