Borrelia Species DNA, Real‑Time PCR, with Reflexes, Synovial Fluid/CSF
Also known as: Lyme, Borreliosis, Tick borne
Use
The diagnosis of Borreliosis is most often made by clinical examination combined with evidence of tick bite or exposure in endemic areas. Amplification of Borrelia genomic DNA from synovial fluid or cerebrospinal fluid can support the diagnosis. This test detects Borrelia species associated with either Lyme disease (Borrelia burgdorferi) or relapsing fever (Borrelia miyamotoi). A positive result for Borrelia species but negative results for B. burgdorferi and B. miyamotoi may indicate that other Borrelia species caused infection. A negative PCR result does not rule out infection, and appropriate serology may be clinically warranted.
Special Instructions
Not provided.
Limitations
Not provided.
Methodology
PCR-based (PCR)
Biomarkers
Result Turnaround Time
2-3 days
Related Documents
For more information, please review the documents below
Specimen
Cerebrospinal Fluid
Volume
3 mL
Minimum Volume
0.5 mL
Container
Sterile, plastic, leak‑proof container
Collection Instructions
3 mL (0.5 mL minimum) CSF or synovial fluid collected in a sterile, plastic, leak‑proof container
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 7 days |
| Frozen | 30 days |
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