Broad Range Bacterial PCR and Sequencing, Varies
Use
This test is used for detecting and identifying bacteria, including mycobacteria, from normally sterile sources. It is particularly useful for identifying bacteria in specimens where they are visible but other methods have failed. The test is not recommended as a test of cure since nucleic acids may persist after successful treatment. It is optimal for detecting fastidious or slow-growing bacterial infections that do not yield a culture.
Special Instructions
Not provided.
Limitations
The test does not detect nonbacterial organisms like viruses, fungi, helminths, or protozoa but will detect mycobacteria. False-positive results can occur due to contamination from the environment or skin microbiota. In some cases, sequencing could have extended turnaround times if additional testing is required. Bacterial nucleic acids may persist for long periods, complicating post-treatment assessments.
New York Approved
Methodology
PCR-based (qPCR)
Biomarkers
Bacteria (including Mycobacteria)
Microorganism
LOINC Codes
- 76575-0 - Bact 16S rRNA Spec Ql NAA+probe
- 76575-0 - Bact 16S rRNA Spec Ql NAA+probe
Result Turnaround Time
14-28 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Entire collection or 5 mm(3)
Minimum Volume
5 mm(3)
Container
Sterile container
Collection Instructions
1. Collect fresh tissue specimen. 2. Submit tissue only, do not add fluid to tissue. 3. Freeze specimen.
Storage Instructions
Frozen (preferred) <21 days/Refrigerated <21 days
Causes for Rejection
Specimen received in anaerobe vial, tissue received in any fluid (saline, broth, formalin, formaldehyde, acetone, etc)
Stability Requirements
| Temperature | Period |
|---|---|
| Frozen | <21 days |