CD30, technical only
Use
CD30 is a transmembrane, type I, glycoprotein receptor from the tumor necrosis factor receptor (TNFR) superfamily that interacts with the CD30L cytokine ligand. CD30 antigen is reported to be expressed on the surface of multinucleated Reed Sternberg cells, classic Hodgkin lymphoma, anaplastic large cell lymphoma and subtypes, and virally transformed cells (e.g., EBV-transformed B-cells). The test provides important information related to these diseases. However, it is technical component only; pathologist read not included.
Special Instructions
Not provided.
Limitations
This test has technical constraints as it includes only the technical component without pathologist interpretation. Proper fixation in 10% neutral buffered formalin is essential for accurate results, and the use of non-standard fixatives will lead to specimen rejection. The precise read time following ethanol transfer must be within 3 months to ensure valid results.
Methodology
Immunoassay (IHC)
Biomarkers
CD30
Protein
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours preferred). Unstained slides should be formalin-fixed.
Storage Instructions
Store at 15°C to 25°C. Use a refrigerated (not frozen) gel pack during shipment to protect from extreme temperatures and separate it from the specimen.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus; all other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours (Fresh Tissues), Indefinite (FFPE and unstained slides) |