CD31 by Immunohistochemistry

Casandra

Casandra Test Code AR33215Version 1 (DRAFT)

Performing Lab

Lab ARUP LaboratoriesLab Test ID 2003550CPT Code 88342

CD31 by Immunohistochemistry

Also known as: CD31 IHC

Clinical Use

Order Test

Use

CD31 immunohistochemistry (IHC) is a stain-only technical service used to highlight endothelial-lined structures by targeting CD31, a transmembrane glycoprotein commonly present on vascular endothelial cells, megakaryocytes, histiocytes, and plasma cell subsets. It is utilized to assist in the identification and characterization of vascular neoplasms and endothelial cell involvement within histologic specimens. This service does not include diagnostic interpretation and is intended for laboratories that perform their own slide review.

Special Instructions

Not provided.

Limitations

Specimens fixed and embedded incorrectly may yield suboptimal staining. Frozen specimens are unacceptable. Depleted specimens or those with non-representative tissue types are unacceptable. Slides must be prepared correctly (formalin-fixed paraffin-embedded cellblocks or tissue), with positively charged slides (3–5 µm thick; minimum of two slides). Precautions must be taken to avoid excessive heat and slide baking. Transport in cooled container during summer months; frozen transport is unacceptable.

Test Details

New York Approved

Methodology

Immunoassay (IHC)

Biomarkers

2 components

CD31 by IHC • IHCCD31 Reference Number • IHC

LOINC Codes

Result Codes

94736-6 - Tissue block ID Spec

Result Turnaround Time

1-3 days

Specimen

Tissue (FFPE)

Volume

Not provided

Minimum Volume

5 unstained (3- to 5-micron) slides (minimum 2 slides)

Container

Tissue transport kit (ARUP supply #47808)

Collection Instructions

Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen; cells must be prepared into a cellblock or tissue block. Transport tissue block or 5 unstained (3–5‑micron thick), positively charged slides. If sending precut slides, do not oven bake.