Cell Surface (Epithelial) Antibodies, IgG by IIF

Casandra

Casandra Test Code AR78846Version 1 (DRAFT)

Performing Lab

Lab ARUP LaboratoriesLab Test ID 0090266CPT Codes 88346, 88350

Cell Surface (Epithelial) Antibodies, IgG by IIF

Also known as: IGG EPI AB

Clinical Use

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Use

This test assesses and monitors cell surface (CS) antibodies, also known as intercellular substance (ICS) antibodies, in patients with IgG pemphigus variants. It is useful for cases where normal IgG desmoglein 1 and IgG desmoglein 3 antibody levels are present. The testing is recommended to be correlated with concurrent Direct Immunofluorescence on a tissue biopsy, as CS/ICS antibodies by indirect immunofluorescence are commonly positive in IgG pemphigus variants, including pemphigus foliaceus and pemphigus vulgaris.

Special Instructions

Testing should initially be correlated with concurrent Direct Immunofluorescence, Tissue Biopsy (Cutaneous, Mucosal, Epithelial) (0092572). The Serum Pemphigus Antibody Panel, IgG (0090650) has increased diagnostic sensitivity and specificity for pemphigus. For comprehensive testing, consider ordering the Immunobullous Disease Antibody Panel (3001409).

Limitations

This test does not exclude the diagnosis of pemphigus vulgaris, pemphigus foliaceus, or another IgG pemphigus variant if negative. Indirect immunofluorescence testing results need clinical correlation, including direct immunofluorescence findings and treatment status. Cell surface reactivity may be observed transiently and/or nonspecifically in normal individuals or in cases of drug reactions, infections, and other mucocutaneous diseases.

Test Details

New York Approved

Methodology

Immunoassay (Indirect Immunofluorescence)

Biomarkers

IgG Epithelial Cell Surface

Protein

IIF • Expression • Ordinal Score (e.g., TPS, CPS, H-score)

Result Turnaround Time

3-9 days

Specimen

Serum

Volume

2 mL

Minimum Volume

0.5 mL

Container

ARUP standard transport tube

Collection Instructions

Transfer 2 mL serum to an ARUP standard transport tube.

Storage Instructions

Store refrigerated unless shipping promptly (within 2 hours). Transport at ambient temperature to arrive within 7 days. If 7-14 days until received in laboratory, store and ship refrigerated. If greater than 14 days, serum specimens must be stored and shipped frozen.