Cortisol

Casandra

Casandra Test Code LC40394Version 1 (DRAFT)

Performing Lab

Lab LabcorpLab Test ID 004051CPT Code 82533

Also known as: Compound F, Hydrocortisone

Clinical Use

Order Test

Use

Establish the diagnosis of adrenocortical insufficiency, Addison's disease, adrenocortical hypersecretion, Cushing's syndrome. Malfunction of the organs in the hypothalamic-pituitary-adrenal cortex axis will result in alteration of the cortisol levels.

Special Instructions

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin. It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

Limitations

Pregnancy, contraceptives, and estrogen therapy give rise to elevated cortisol concentrations. In samples from patients who have been treated with prednisolone, methylprednisolone, or prednisone, falsely elevated concentrations of cortisol may be determined. During metyrapone tests, 11-deoxycortisol levels are elevated. Falsely-elevated cortisol values may be determined due to cross reactions. Patients suffering from 21-hydroxylase-deficiency exhibit elevated 21-deoxycortisol levels which can cause elevated cortisol levels. The time of sample collection must be taken into account when interpreting results due to the cortisol secretion circadian rhythm. Severe stress and treatments with monoclonal mouse antibodies can also interfere with results.

Test Details

Methodology

Immunoassay (ECLIA)

Biomarkers

LOINC Codes

Order Codes

2143-6

Result Codes

2143-6

Result Turnaround Time

1 day

Specimen

Serum

Volume

1 mL

Minimum Volume

0.7 mL

Container

Gel-barrier tube (preferred) or red-top tube

Collection Instructions

If a red-top tube is used, transfer separated serum to a plastic transport tube. Blood should be drawn at 8 a.m. and 4 p.m. to evaluate baseline diurnal variation.