Cyclin-dependent kinase-4, technical only
Use
Cyclin-dependent kinase-4 (CDK4) is a cyclin-dependent kinase that associates with D-type cyclins. It plays a significant role in the progression of cells from G1-phase to S-phase of the cell cycle. This antibody does not cross-react with other members of the CDK family. CDK4 may be used to differentiate benign tumors from sarcomas and osteosarcomas. This test includes only the technical component; a pathologist read is not included.
Special Instructions
Not provided.
Limitations
The antibody is specific to CDK4 and does not cross-react with other CDK family members. The test is limited to technical analysis only and does not include interpretive pathology. Specimens must be fixed in 10% NBF or B plus, as other fixatives will result in rejection. Specimen stability varies, with fresh tissues stable for up to 72 hours, while FFPE and slides have indefinite stability.
Methodology
Immunoassay (IHC)
Biomarkers
Cyclin-dependent kinase-4
Protein
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be 20:1 fixative to tissue ratio using 10% neutral buffered formalin (NBF) for 6-72 hours, preferably 48-72 hours. Specimens may be transferred to 70% alcohol for transportation after fixation.
Storage Instructions
Store at ambient temperature. For longer-term storage, specimens fixed in formalin may be transferred to 70% ethanol and stored at 2-8°C.
Causes for Rejection
Specimens not fixed in 10% NBF or B plus will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |
| Refrigerated | Indefinite |
| Frozen | Indefinite |