Cytomegalovirus by Qualitative PCR

Casandra

Casandra Test Code AR32485Version 1 (DRAFT)

Performing Lab

Lab ARUP LaboratoriesLab Test ID 0060040CPT Code 87496

Cytomegalovirus by Qualitative PCR

Also known as: CMVPCR

Clinical Use

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Use

The qualitative detection of cytomegalovirus (CMV) DNA by polymerase chain reaction (PCR) is used to diagnose CMV infection. This test is beneficial for identifying CMV presence in patients where quantification is not required. It is particularly useful for screening in bone marrow transplants, detecting congenital infections, and diagnosing CMV-related complications in immunocompromised individuals. This test does not provide viral load quantification; if quantification is needed, refer to other specific tests.

Special Instructions

The test should not be used for viral load assessment. Proper specimen collection is necessary to avoid DNA contamination, and source labeling is crucial. Inappropriate samples like heparinized blood or suboptimal tissue samples can lead to rejection of the specimen. Compliance with the provided storage and transportation guidelines must be followed to ensure the validity of the test results.

Limitations

This test detects the presence of CMV DNA but does not quantify the viral load, which limits its use in monitoring disease progression or response to treatment where viral load is relevant. It might not detect the virus in all cases, particularly when viral DNA is present below the detection level or when PCR inhibitors are present in the sample. Therefore, a negative result does not definitively exclude the presence of CMV. Heparinized specimens and tissues in optimal cutting temperature compound are not acceptable as they can interfere with the PCR process.

Test Details

New York Approved