Dengue Virus, Molecular Detection, PCR, Serum
Use
Dengue virus (DV) is a globally distributed flavivirus with 4 distinct serotypes (DV-1, -2, -3, -4) primarily transmitted by the Aedes aegypti mosquito. Detecting DV nucleic acid in serum marks an acute infection. The test differentiates serotypes and helps in diagnosing acute infections, important for patient management in endemic areas.
Special Instructions
The nucleic acid detection may overlap with dengue virus nonstructural protein 1 (NS1) antigen presence. For patients with symptoms lasting more than 1 week, serologic testing is advised to confirm DV infection.
Limitations
The window for virus detection in serum via molecular testing is brief; hence, testing should occur within the first week of symptom onset. After this period, serologic testing is preferred. False negatives may occur if the specimen is collected more than 7 days after symptom onset.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 7855-0
- 7855-0
Result Turnaround Time
1-5 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.3 mL
Container
Sterile container
Collection Instructions
Within 2 hours of collection, centrifuge and aliquot the serum into a sterile container.
Causes for Rejection
Serum specimens not aliquoted from the serum gel collection tube into a sterile container will be rejected. Reject if gross hemolysis or heat-inactivated specimen.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |