Direct Antiglobulin Test (Polyspecific), Blood
Use
The Direct Antiglobulin Test (DAT) is used to demonstrate in vivo coating of red blood cells with immunoglobulin G (IgG) or complement component C3d. This test is valuable in identifying immune-related causes of hemolysis in patients, such as autoimmune hemolytic anemia, hemolytic transfusion reactions, and drug-induced hemolytic anemia. Positive results can indicate the presence of IgG or complement on the surface of erythrocytes, which may lead to hemolysis.
Special Instructions
The specimen must arrive at the laboratory within 48 hours of collection to ensure the validity of the test results. It is important to properly mix the blood by inverting the tube several times after collection. Additionally, the blood sample should be sent in its original tube without aliquoting.
Limitations
The test may not detect all cases of autoimmune hemolytic anemia, as some patients with the condition can have negative DAT results. Hemolysis can occur in some individuals who may have positive DAT results yet exhibit no apparent clinical symptoms. False negatives may occur if the specimen is not received within the specified timeframe or if there are improper handling or storage conditions.
FDA Approved
Methodology
Other
Biomarkers
LOINC Codes
- 1007-4
- 1007-4
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
6 mL
Minimum Volume
3 mL; Neonates: EDTA Micro tube 0.5 mL
Container
Pink top (EDTA)
Collection Instructions
Invert several times to mix blood. Send whole blood specimen in original tube. Do not aliquot.
Causes for Rejection
Gross hemolysis will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |
| Refrigerated | 48 hours |