E. coli Shiga-like Toxin by EIA
Also known as: ECSLT AG
Use
The test detects Shiga toxins, which are sensitive markers of toxigenic Escherichia coli. It is widely recommended by the CDC to culture stool specimens for E. coli O157:H7 (O157 STEC) when performing this test. Detection of Shiga-like toxins is critical as these toxins may be produced by numerous serotypes of E. coli as well as by Shigella dysenteriae type 1 strains. The level of toxin does not correlate with the severity of disease, and a positive result does not exclude infection caused by another enteric pathogen.
Special Instructions
Place 5 mL stool in enteric transport media (Cary-Blair) immediately after collection. The enteric transport is available through ARUP Supply or by contacting ARUP Client Services. Refrigerate stool specimens for transport to ensure sample stability. The test is performed every day of the week, from Sunday to Saturday.
Limitations
This test identifies the presence of E. coli Shiga-like toxin but cannot determine specific strains of E. coli, such as E. coli O157:H7. A negative test result does not exclude the diagnosis of hemorrhagic colitis.
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 21262-1
Result Turnaround Time
1-2 days
Related Documents
For more information, please review the documents below
Specimen
Stool
Volume
5 mL
Minimum Volume
1 mL
Container
enteric transport media (Cary-Blair)
Collection Instructions
Place 5 mL stool in enteric transport media immediately after collection.
Storage Instructions
Refrigerated
Causes for Rejection
Specimens other than stool.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 1 hour |
| Refrigerated | 72 hours |
| Frozen | 1 week |
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