EGFR 7p12 Amplification, paraffin

Casandra

Casandra Test Code MP95635Version 1 (DRAFT)

Performing Lab

Lab MPLNLab Test ID FP EGFRCPT Code 88377

EGFR 7p12 Amplification, paraffin

Clinical Use

Use

FISH testing on paraffin-embedded cells for detection of EGFR (7p11.2) gene amplification. Amplification of EGFR is a recurrent abnormality in a variety of cancers, including non-small cell lung cancer, metastatic colorectal cancer, and glioblastoma. The detection of EGFR amplification can help in the diagnosis and potential therapeutic targeting of such cancers.

Special Instructions

Tissues should be fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually not suitable for FISH. Assay requires 3 unstained slides minimum (two for each probe) and one H&E stained slide.

Limitations

Assay accuracy may be compromised if tissues are not properly fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually unsuitable for FISH analysis. Improper fixation and extreme temperatures can lead to specimen rejection. The test is not validated for tissues fixed with alternative methods.

Test Details

Methodology

Chromosomal / Cytogenetics (FISH)

Biomarkers

EGFR

Gene

Amplification • Copy Number Alteration • Categorical (e.g., Positive / Negative / Indeterminate)

Result Turnaround Time

9 days

Specimen

Tissue (FFPE)

Volume

Not provided

Minimum Volume

3 slides

Container

positively charged slides

Collection Instructions

Cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides. Use at least 3 unstained slides; two for each probe and one H&E stained slide.