EGFR Mutation Analysis by PCR
Also known as: EGFR, epidermal growth factor receptor
Use
This assay is designed to detect highly recurrent EGFR alterations in exons 18–21—specifically Exon 18 G719X; Exon 19 deletions; Exon 20 T790M, C797S, Exon 20 insertions and S768I; Exon 21 L858R, L861Q—enabling identification of key activating and resistance mutations in EGFR relevant for guiding targeted therapy decisions in oncology.
Special Instructions
In order to obtain the full list of detectable EGFR variants beyond the highly recurrent ones listed, the client should contact NeoGenomics client services.
Limitations
The assay does not detect some less common EGFR mutations beyond the recurrent alterations listed in exons 18–21; clients must contact NeoGenomics client services for a full list of detectable variants.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
Result Turnaround Time
7 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Paraffin block preferred; alternatively send 1 H&E slide plus 5–10 unstained slides cut at ≥5 microns; use positively‑charged slides and 10% NBF fixative; do not use zinc fixatives.
Storage Instructions
Use cold pack for transport, ensuring the cold pack is not in direct contact with specimen. For NYS clients, provide date and time of collection.