Epstein-Barr Virus DNA Detection and Quantification, Plasma
Use
This test is useful for diagnosing Epstein-Barr virus (EBV)-associated infectious mononucleosis, especially in individuals with equivocal or discordant EBV serologic marker test results. It also aids in diagnosing post-transplant lymphoproliferative disorders (PTLD), particularly in EBV-seronegative organ transplant recipients receiving antilymphocyte globulin for induction immunosuppression and OKT-3 treatment for early organ rejection. Additionally, it is used for monitoring the progression of EBV-associated PTLD in organ transplant recipients. Note that this test should not be used to screen asymptomatic patients.
Special Instructions
The test is calibrated to the First World Health Organization International Standard for EBV for nucleic acid amplification techniques, ensuring accurate quantification of EBV DNA levels in plasma. Proper specimen collection involves centrifuging and aliquoting plasma into a plastic vial, followed by freezing for shipment. Ensure continuous monitoring of EBV DNA levels using the same assay to maintain consistency in results.
Limitations
The assay's quantification range is between 35 and 100,000,000 IU/mL, with a limit of detection at 19 IU/mL. "Undetected" results indicate no EBV DNA presence, and results of "<35 IU/mL" signify detection levels below the quantification threshold. Variability in assay performance necessitates repeated testing for conclusive results, especially in cases of inconclusive findings due to potential PCR inhibitors or interfering substances. Serial determinations are critical to monitor potential PTLD development or treatment efficacy.
FDA ApprovedNew York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 43730-1
- 43730-1
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Plasma
Volume
1.5 mL
Minimum Volume
0.5 mL
Container
Plastic vial
Collection Instructions
Centrifuge blood collection tube and aliquot plasma into a plastic vial per manufacturer's instructions. Freeze aliquoted plasma for shipment.
Storage Instructions
Ship specimen frozen on dry ice only. If shipment will be delayed for more than 24 hours, freeze plasma at -20 to -80 degrees C (up to 84 days) until shipment on dry ice.
Causes for Rejection
Gross hemolysis
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 6 days |
| Frozen | 84 days |