Epstein-Barr Virus (EBV) by in situ Hybridization on Paraffin
Also known as: EBVP ISH
Use
The test is used to detect Epstein-Barr virus (EBV) in paraffin-embedded tissue samples. It utilizes in situ hybridization (ISH) to identify the presence of EBV Messenger RNA (mRNA), which helps diagnose EBV-associated diseases. This method is highly specific for detecting EBV in tissues.
Special Instructions
Include a surgical pathology report with the specimen. Ensure that tissue blocks or slides are adequately protected from excessive heat during transport. Specimens need to be accompanied by clear instructions regarding which sample should be tested if multiple specimens are submitted. A Pathologist Block Selection Fee might apply for selecting the best specimen for testing.
Limitations
The performance characteristics of this test have been determined by ARUP Laboratories in a CLIA certified environment. However, the test has not been cleared or approved by the FDA. The test's accuracy is contingent upon proper specimen preparation, handling, and storage. Specimens not fixed in 10% neutral buffered formalin or undergoing decalcification processes could lead to inaccurate results. The test is unsuitable for tissue without remaining tumor.
New York Approved
Methodology
Chromosomal / Cytogenetics (ISH)
Biomarkers
Result Turnaround Time
2-5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
4 slides
Collection Instructions
Formalin-fix (10% neutral buffered formalin) and paraffin-embed tissue. Transport as tissue block or unstained slides.
Storage Instructions
Protect from excessive heat; transport in cooled container during summer months.
Causes for Rejection
No tumor tissue remaining in paraffin block; specimens fixed in non-formalin fixatives; decalcified specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |
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