Estradiol, Free
Use
This test measures both free estradiol and total estradiol concentrations in serum. The free (unbound) fraction may better correlate with clinical conditions such as infertility, menopause, polycystic ovary syndrome, and other endocrine disorders due to its active nature compared to total estradiol. It is used in the evaluation of hormone-related disorders. Source: Includes both free and total estradiol ([reddit.com](https://www.reddit.com/r/DrWillPowers/comments/zgfaoz?utm_source=openai))
Special Instructions
Specify age and sex on the test request form. Simple red-top (plain) tube required; avoid serum separator tubes. Testing is performed on Mondays and Wednesdays. Source: quest instructions and specimen requirements ([intellitestmanager.questdiagnostics.com](https://intellitestmanager.questdiagnostics.com/home/output/220754?utm_source=openai))
Limitations
This assay was developed and analytically validated by Quest Diagnostics under CLIA regulations and is not cleared or approved by the FDA. Be aware of possible interferences: reject samples in serum separator tubes; also affected by hemolysis, lipemia, grossly icteric specimens. Source: Limitations and regulatory status ([jdos.nicholsinstitute.com](https://jdos.nicholsinstitute.com/dos/Medfusion/test/543175?utm_source=openai))
Methodology
Mass Spectrometry
Biomarkers
Result Turnaround Time
3-9 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
2.5 mL (Minimum: 1.5 mL) aliquoted serum
Minimum Volume
1.5 mL
Container
Plain red-top tube (no gel)
Collection Instructions
Centrifuge plain red, aliquot 2.5 mL serum
Causes for Rejection
Serum separator tube; hemolysis; grossly icteric; lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 24 hours |
| Refrigerated | 7 days |
| Frozen | 28 days |
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