Fumarate Hydratase by Immunohistochemistry
Also known as: FUMHYD IHC
Use
Fumarate Hydratase by Immunohistochemistry (IHC) is performed to qualitatively assess the presence of fumarate hydratase protein in tissue samples. This test is primarily used to aid in the diagnosis of fumarate hydratase deficiency and possibly evaluate FH-deficient tumors. Fumarate hydratase deficiency is associated with hereditary leiomyomatosis and renal cell cancer (HLRCC). The presence or absence of fumarate hydratase protein may assist in the evaluation of certain tumor syndromes. It is intended for clinical purposes and performed in a CLIA certified laboratory.
Special Instructions
Not provided.
Limitations
This lab-developed test has not been cleared or approved by the FDA. As an immunohistochemistry-based test, potential limitations include pre-analytical variations such as tissue fixation, antigen retrieval methods, and the inherent subjectivity in IHC interpretation. Staining results may also be affected by the degradation of tissue antigenicity over time if not properly fixed or stored. Conditions submitted with non-representative tissue type or depleted specimens will be rejected, which further limits the applicability of testing certain sample types.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Fumarate Hydratase
Protein
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
2 slides
Container
Paraffin block or 5 unstained positively charged slides
Collection Instructions
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen. Protect paraffin block and/or slides from excessive heat. Transport tissue block or slides in a tissue transport kit. Do not oven bake precut slides.
Causes for Rejection
Non-representative tissue type or depleted specimens.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinitely |
| Refrigerated | Indefinitely |
| Frozen | Unacceptable |
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